Denaturing, annealing, and extending.
Well it’s amazing what heating and cooling and heating will do
And the chorus:
PCR, when you need to detect mutations.
PCR, when you need to recombine.
PCR, when you need to find out who the daddy is.
PCR, when you need to solve a crime
Disclaimer: This is in no way an endorsement of Bio-Rad, although I do use some of their products because they have me trapped into a dependancy cycle.